The BIObus is the mobile field research vehicle of the Centre for Biodiversity Genomics (CBG), located at the University of Guelph in south-western Ontario, Canada. From 2008-2020, the BIObus carried researchers and students to biodiversity hotspots across North America to collect insects and other invertebrates. It has been to all of the Canadian provinces and one territory and several parts of the United States. Hundreds of thousands of specimens representing over 20,000 species were collected. Samples were sorted, labeled, and tissue sampled. DNA barcodes are obtained through DNA sequencing. Any new species to the Barcode of Life library (BOLD) are photographed.